Plan a long-read sequencing workflow appropriate to a research question
Life, Earth & Environmental Sciences · Biological Sciences & Genetics
GEN-360Long-Read Sequencing & Nanopore Analysis
Covers long-read sequencing workflows from sample and library considerations through basecalling, quality control, alignment, variant analysis, and reproducible reporting.
Course outcomes
What students develop
Evaluate read quality, yield, length, and contamination
Run documented alignment and analysis pipelines
Communicate analytical limitations and data-governance requirements
Course content
Topics and questions
- Nanopore sequencing principles
- Library and run design
- Basecalling and quality control
- Alignment, assembly, and variant analysis
- Reproducibility and data stewardship
Methods & resources
Tools and environments
- Long-read sequencing platform
- Research computing environment
- Bioinformatics command-line and notebook tools
Evaluation
Demonstration of learning
- Run plan
- Quality-control report
- Analysis pipeline
- Final technical report
Program relationships
Where this course fits
Core
Integrative Biosciences & Genomic Methods
Connects biological foundations to genetics, genomics, evolution, physiology, ecology, computation, and laboratory practice.
Core
Genomics, Epigenetics & Biological Systems
Advanced study in genomic variation, gene regulation, long-read sequencing, bioinformatics, environment, and biological systems.
Core
Nanopore Genomics & Long-Read Analysis
Long-read sequencing, quality control, alignment, assembly, variant analysis, reproducibility, and genomic data stewardship.
Core
Long-Read Genomics
Run planning, long-read sequencing, quality control, alignment, analysis, reproducibility, and technical reporting.
Course inquiry
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